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KMID : 0379120110390030243
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Korean Journal of Mycology
2011 Volume.39 No. 3 p.243 ~ p.248
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Bioethanol Production using a Yeast Pichia stipitis from the Hydrolysate of Ulva pertusa Kjellman
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Lee Ji-Eun
Lee Sang-Eun
Choi Woon-Yong
Kang Do-Hyung
Lee Hyeon-Yong
Jung Kyung-Hwan
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Abstract
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We studied the repeated-batch process for the bioethanol production from the hydrolysate of Ulva pertusa Kjellman using yeast Pichia stipitis, which is able to assimilate C6- and C5-monosaccharides. During 180-hour operations, the repeated-batch process was carried out stably, and the average bioethanol concentration reached 11.9 g/L from about 30 g/L of reducing sugar in the hydrolysate. Meanwhile, the bioethanol yields, based on the reducing sugar and the quantitative TLC analysis, were 0.40 and 0.37, respectively, which corresponded to 78.4% and 72.5% of theoretical value, respectively. Throughout the quantitative process analysis, it was also demonstrated that 39.67 g-bioethanol could be produced from 1 kg of dried Ulva pertusa Kjellman. In this study, we verified that the bioethanol production from the hydrolysate of Ulva pertusa Kjellman was feasible using a yeast Pichia stipitis, particularly during the repeated-batch operation.
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KEYWORD
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Bioethanol, Pichia stipitis, Repeated-batch, Ulva pertusa Kjellman
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